Micropipetting Procedure
Learning Outocme #3
- Prepares reagents, standards and solutions to industry standards for use in the clinical lab, utilizing common lab equipment according to established protocols.
Supplies
- Gloves and protective eyewear
- Deionized water
- Pipettor
- Pipettor tips
- Kimwipes
- Beakers
Instructions
Read the list of required supplies and follow the procedure steps.
Follow the procedure in the correct order of steps.
- Attached the proper tip for the micropipette.
- Press to the stop position.
- Place the tip into the solution and allow the piston to rise slowly back to its original position. (This fills the pipettor tip with the desired volume of solution.
- Do not wipe the tip.
- Place the tip on the wall of the receiving vessel and depress the piston, first to a stop position where the liquid is allowed to drain, then to a second stop position where the full dispensing of the liquid takes place.
- Press down to the last stop and dispose of the tip in the benchtop biohazard bucket.