Procedure for setting Kohler Illumination

Instructions

Follow the procedure below in the exact order.

1. Place a slide on the stage and focus the object using the low-power objective (10x).
2. Ensure the condenser is completely raised by turning the condenser focus know toward the back of the microscope.
3. Close the field diaphragm.
4. Using the condenser focus know, lower the condenser until the edges of the field diaphragm are sharply focused.
5. Center the image of the field diaphragm using the condenser centering adjustment screws.
6. Open the field diaphragm until its image is at the edge of the field. Then open it slightly more so the image is just beyond the edge.