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Appendix C Microscopy Troubleshooting

Issue Possible Cause
No light from light source Power switch off
Power cord improperly connected or not plugged in
Problem with power supply
Blown fuse
Faulty bulb/defective bulb contacts
No light seen through microscope objectives Light source covered
Field diaphragm closed
Aperture/iris/condenser diaphragm closed
Objective not in the light path
Field only partially illuminated Condenser off-center
Aperture/iris/condenser diaphragm closed too much for the objective being used
Field diaphragm partially closed
Objective only partly in light path
Electrical cord lying in the field
For Nikon 50i: neutral density filter partly pushed in
For Nikon 50i: optical path switching lever (for camera) in incorrect position
Weak illumination Light adjustment knob/rheostat/lamp voltage turned too low
Light source covered or burning out
Aperture/iris/condenser diaphragm closed too much for the objective being used
Condenser too low or not centered
Using oil immersion lens with no oil
For Nikon 50i: neutral density filter engaged
For Nikon 50i: preset switch has been engaged (only required for camera use)
Uneven illumination Light source not centered
Condenser not centered
Field of view too bright Light adjustment knob set too high
Image cannot be focused Slide very dirty/greasy
Slide up-side down
All objectives dirty
Dirty eyepieces
Diopter adjustment improperly set
Vibration on the bench
Image very yellow Blue filter missing
Light adjustment knob set too low

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