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About this Manual
1. Transfusion Medicine Lab Guidelines
2. Laboratory Evaluation Rubric
3. Professional Conduct Rubric
4. Nova Scotia Community College Medical Laboratory Technology Program
5. Transfusion Medicine Student Cupboard Supplies
6. Cell Washers Information and Troubleshooting
7. Cell Washer Maintenance Charts
8. Inverted Microscope Parts and Information
9. Inverted Microscope Maintenance Charts
10. Quality Control Worksheet
11. Dry Bath Maintenance Chart
12. Specimen Acceptance/Separation/Labeling/Storage
13. Patient History Check
14. Patient Records
15. Tube Labeling
16. Board / Rack Set-Up
17. Reading, Grading, and Recording Reactions
18. Preparation of Red Cell Suspensions
19. Cell Washing: Manual Method
20. Cell Washing: Automated Method
21. Tube Tests for ABO Typing
22. Manufacturer’s Instructions for Phenotyping Red Cells
23. Tube Tests for Rh Typing of Red Cells
24. Phenotyping Worksheet
25. Phenotyping - “Indirect” Method
26. Phenotyping -“Direct” Method
27. ABO and Rh Worksheet
28. Testing for Weak D
29. Prediction of Rh Genotypes from Phenotypes
30. Saliva Testing for ABH Substances
31. Saline Replacement for Rouleaux
32. Resolving ABO Anomalies
33. ABO Worksheet
34. Direct Antiglobulin Test (DAT)
35. Antibody Detection and Identification Flowchart
36. Group/Screen (ABS) and BB Hold Procedure
37. Antibody Screening by Saline IAT (SIAT)
38. Antibody Screening by LISS IAT
39. Antibody Identification - LISS
40. Antibody Screening (ABS) by Gel Method (G-IAT)
41. Antibody Identification (ABID) – Gel Method (G-IAT)
42. Antibody Identification (Matching or Exclusion)
43. Selected Panels (cells)
44. ABS by Micro Typing Systems (MTS)-Extended
45. ABID by Micro Typing Systems (MTS)- extended
46. MTS Maintenance Chart
47. Antibody Identification, Exclusions and Confirmations…
48. Crossmatching Flow Chart
49. LISS Antihuman Globulin (AHG) Crossmatch
50. Crossmatch GEL-MTS Method
51. Immediate-spin Crossmatch
52. Pre-warming Technique
53. GammaZyme-B Bromelin Solution™
54. Fetal Maternal Testing Flow Chart
55. Prenatal Screening
56. Rh Immune Globulin (RhIg)
57. A Mini Panel for Passive Anti-D (RhIg)
58. Cord Specimens
59. Postnatal and Cord lab Instructions
60. Antibody Titration
61. Fetal Bleed Screening Test
62. Kleihauer-Betke Test (Modified) (using a “Simmler” kit)
63. Acid Elution (ELU KITTM PLUS)
64. WAIHA Elutions using Warm Autoantibody Removal Medium (W.A.R.M.)
65. “General” Warm-Reactive Autoantibody Adsorption Procedure
66. Investigation of Adverse Effects of Transfusion
67. Exercise on ABO/Rh groupings
68. ABO Discrepancies/Anomalies Exercise
69. Student Exercise on unexpected antibodies
70. “Oscar F” Case Study Exercise
71. Problem Solving Exercise on Antibody Identification
72. Mom and Baby Exercise
73. Elution Student Questions
74. Problem Solving Exercise on DAT’s and Elutions
75. Problem Solving Exercise on Transfusion Reactions
76. Lab Summary - Student Assessment
References
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