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About the Manual
Professional Conduct Rubric
Laboratory Evaluation Rubric
General Safety Precautions and Guidelines
Notes
Safety Information on Contact Lenses
Duties-Histotechnology Lab Rotation
Sample Histology Requisition
Accessioning Tissue Specimens
Histotechnology Report Form
Grossing Tissues
Decalcification and End Point Detection of Calcified Tissues
Operating the Tissue Processor
Processor Maintenance Form
Recycling of Reagents
FormaSolve Recycler
Embedding tissues in Paraffin Wax
Embedding Orientation
Self-Evaluation of Embedding
Microtomy
Microtomy Procedure
Levels, Serial Sections and Deepers
Troubleshooting Microtomy
Self-Evaluation for Microtomy
Cryotomy (Frozen Sections)
Leica CM1520 Cryostat
Procedure for Frozen Sections
Cryotomy Troubleshooting
Staining of Tissue Slides
Mounting Slides with Permount
Routine Hematoxylin and Eosin (H&E): Regressive Method
Rapid Hematoxylin & Eosin (Progressive Method)
Self-Evaluation H&E
Special Staining Techniques: Lab Staining Report
Acid Solutions for Cleaning Glassware
Quality Control Record Sheet for QC Slides
Massons Trichrome
Gomori's Silver Impregnation Method for Reticulin Fibers Argyrophilic method
Verhoeff-van Gieson Method for Elastic Fibers
Periodic Acid-Schiff (PAS), +/- Diastase Digestion
Alcian Blue pH 2.5
Gomori's Silver Methenamine Impregnation for Fungi*
Perl’s Prussian Blue for Ferric Iron
Methods for Lipid Demonstration: Oil Red O
Immmunohistochemical (IHC) Methods: Immunoperoxidase Staining
References
MLT Lab Rubric for Fall 2022
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